SEC5/EXOC2 Polyklonaler Antikörper

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• KD/KO Validated

SEC5/EXOC2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12751-1-PBS

Synonyme

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  WB analysis of mouse brain using 12751-1-AP (same clone as 12751-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12751-1-AP (same clone as 12751-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human ileum using 12751-1-AP (same clone as 12751-1-PBS)

  human ileum tissue were subjected to SDS PAGE followed by western blot with 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 12751-1-AP (same clone as 12751-1-PBS)

  IP result of anti-SEC5/EXOC2 (IP:12751-1-AP, 5ug; Detection:12751-1-AP 1:500) with mouse brain tissue lysate 3000ug. This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12751-1-AP (same clone as 12751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12751-1-AP (same clone as 12751-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12751-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12751-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA SEC5/EXOC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEC5/EXOC2 fusion protein Ag3147
• Vollständiger Name: exocyst complex component 2
• Berechnetes Molekulargewicht: 924 aa, 104 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: BC016918
• Gene symbol: SEC5
• Gene ID (NCBI): 55770
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.