SEC6 Polyklonaler Antikörper

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• KD/KO Validated

SEC6 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14703-1-PBS

Synonyme

EXOC3, Exocyst complex component 3, Exocyst complex component Sec6, SEC6L1

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  WB analysis of human brain using 14703-1-AP (same clone as 14703-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:86 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14703-1-AP (same clone as 14703-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14703-1-AP (same clone as 14703-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 14703-1-AP (same clone as 14703-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 14703-1-AP (same clone as 14703-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14703-1-AP (same clone as 14703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 14703-1-AP (same clone as 14703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 14703-1-AP (same clone as 14703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14703-1-AP (SEC6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14703-1-AP (same clone as 14703-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 14703-1-AP (SEC6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14703-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SEC6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEC6 fusion protein Ag6383
• Vollständiger Name: exocyst complex component 3
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 86 kDa
• GenBank-Zugangsnummer: BC064569
• Gene symbol: SEC6
• Gene ID (NCBI): 11336
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Exoc3 (Sec6) is a component of the exocyst (Sec6/8) complex involved in vesicle trafficking at neural synapses and vesicle targeting in polarized epithelial cells. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. This complex is composed of eight proteins (Sec3, Sec5, Sec6, Sec8, Sec10, and Sec15, Exo70 and Exo84), with molecular weights ranging from 70 to 144 kDa.