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SEC61B Polyklonaler Antikörper

SEC61B Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 51020-2-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using SEC61B Polyclonal antibody (51020-2-AP)

    WB analysis using 51020-2-AP (same clone as 51020-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 51020-2-AP (SEC61B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SEC61B Polyclonal antibody (51020-2-AP)

    IHC staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SEC61B Polyclonal antibody (51020-2-AP)

    IHC staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SEC61B Polyclonal antibody (51020-2-AP)

    IHC staining of mouse liver using 51020-2-AP (same clone as 51020-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SEC61B Polyclonal antibody (51020-2-AP)

    IHC staining of mouse liver using 51020-2-AP (same clone as 51020-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using SEC61B Polyclonal antibody (51020-2-AP)

    IF Staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (51020-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using SEC61B Polyclonal antibody (51020-2-AP)

    IF Staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (51020-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SEC61B Polyclonal antibody (51020-2-AP)

    IF Staining of HepG2 using 51020-2-AP (same clone as 51020-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 51020-2-AP (SEC61B antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

51020-2-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA SEC61B und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name Sec61 beta subunit
Berechnetes Molekulargewicht 10 kDa
Beobachtetes Molekulargewicht 10-15 kDa
GenBank-ZugangsnummerBC001734
Gene symbol SEC61B
Gene ID (NCBI) 10952
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).