SEC61G Polyklonaler Antikörper

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• KD/KO Validated

SEC61G Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11147-2-PBS

Synonyme

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  WB analysis of HEK-293 using 11147-2-AP (same clone as 11147-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11147-2-AP (SEC61G antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11147-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 11147-2-AP (same clone as 11147-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11147-2-AP (SEC61G antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11147-2-PBS in a different storage buffer formulation.

• 

  WB analysis of DC2.4 using 11147-2-AP (same clone as 11147-2-PBS)

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 11147-2-AP (SEC61G antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11147-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11147-2-AP (same clone as 11147-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11147-2-AP (SEC61G antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11147-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11147-2-PBS bindet in WB, IHC, Indirect ELISA SEC61G und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEC61G fusion protein Ag1624
• Vollständiger Name: Sec61 gamma subunit
• Berechnetes Molekulargewicht: 7.7 kDa
• Beobachtetes Molekulargewicht: 7.7 kDa
• GenBank-Zugangsnummer: BC009480
• Gene symbol: SEC61G
• Gene ID (NCBI): 23480
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SEC61G is a subunit of the SEC61 complex, which also contains alpha (SEC61A) and beta (SEC61B) subunits. The SEC61 complex is the central component of the protein translocation apparatus on the endoplasmic reticulum membrane. The gene of SEC61G maps to chromosome 7p11.2, and encodes a 68-amino acid single-pass membrane protein. This antibody can recognize endogenous SEC61G that migrates at a molecular mass of ~8 kDa.