SEC63 Polyklonaler Antikörper

SEC63 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28665-1-PBS

Synonyme

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  WB analysis using 28665-1-AP (same clone as 28665-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28665-1-AP (SEC63 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 28665-1-AP (same clone as 28665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 28665-1-AP (same clone as 28665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 28665-1-AP (same clone as 28665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 28665-1-AP (same clone as 28665-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 28665-1-AP (same clone as 28665-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEC63 antibody (28665-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28665-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SEC63 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEC63 fusion protein Ag30213
• Vollständiger Name: SEC63 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC047221
• Gene symbol: SEC63
• Gene ID (NCBI): 11231
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SEC63 is a integral ER membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)