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SEC63 Monoklonaler Antikörper

SEC63 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C10E1

Kat-Nr. : 67352-1-PBS

Synonyme

1C10E1, DnaJ homolog subfamily C member 23, DNAJC23, SEC63L, Translocation protein SEC63 homolog

Filter:
  • Western Blot (WB) analysis of various lysates using SEC63 Monoclonal antibody (67352-1-Ig)

    WB analysis using 67352-1-Ig (same clone as 67352-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67352-1-Ig (SEC63 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67352-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SEC63 Monoclonal antibody (67352-1-Ig)

    IF Staining of HEK-293 using 67352-1-Ig (same clone as 67352-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SEC63 antibody (67352-1-Ig, Clone: 1C10E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67352-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67352-1-PBS bindet in WB, IF/ICC, ELISA SEC63 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen SEC63 fusion protein Ag5783
Vollständiger Name SEC63 homolog (S. cerevisiae)
Berechnetes Molekulargewicht 88 kDa
Beobachtetes Molekulargewicht 90 kDa
GenBank-ZugangsnummerBC047221
Gene symbol SEC63
Gene ID (NCBI) 11231
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SEC63 is a integral ER membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)