P-selectin / CD62P Polyklonaler Antikörper

P-selectin / CD62P Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13304-1-AP

Synonyme

CD62, CD62P, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, P-selectin, SELP

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Galerie der Validierungsdaten

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  WB analysis of human peripheral blood platelets using 13304-1-AP

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13304-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13304-1-AP (P-selectin / CD62P antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Human peripheral blood platelets
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, humanes Lungenkarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below
• FC: See 2 publications below

Produktinformation

13304-1-AP bindet in WB, IHC, IF, FC, ELISA P-selectin / CD62P und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P-selectin / CD62P fusion protein Ag4124
• Vollständiger Name: selectin P (granule membrane protein 140kDa, antigen CD62)
• Berechnetes Molekulargewicht: 830 aa, 91 kDa
• GenBank-Zugangsnummer: BC028067
• Gene symbol: SELP
• Gene ID (NCBI): 6403
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Publikationen

Species	Application	Title
mouse	WB	ACS Appl Mater Interfaces; AIEgen-Based Bionic Nanozymes for the Interventional Photodynamic Therapy-Based Treatment of Orthotopic Colon Cancer.; Authors - Yanhong Duo
human	WB	Int J Biol Macromol; Platelet-membrane camouflaged cerium nanoparticle-embedded gelatin methacryloyl hydrogel for accelerated diabetic wound healing; Authors - Hang Dong
human	FC	World J Gastroenterol; Neutrophil extracellular traps participate in the development of cancer-associated thrombosis in patients with gastric cancer; Authors - Jia-Cheng Li
human	IF,FC	Immun Inflamm Dis; Neutrophil extracellular traps induce thrombogenicity in severe carotid stenosis.; Authors - Shihua Zhang
human	WB	Nat Commun; Ubiquitin ligase CHFR mediated degradation of VE-cadherin through ubiquitylation disrupts endothelial adherens junctions; Authors - Chinnaswamy Tiruppathi

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