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SELPLG/CD162 Rekombinanter Antikörper

SELPLG/CD162 Rekombinant Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241297G10

Kat-Nr. : 84500-1-PBS

Synonyme

SELPLG, 241297G10, CD162, CLA, P-selectin glycoprotein ligand 1

Filter:
  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SELPLG/CD162 Recombinant monoclonal antibody (84500-1-RR)

    IHC staining of human tonsillitis using 84500-1-RR (same clone as 84500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84500-1-RR (SELPLG/CD162 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SELPLG/CD162 Recombinant monoclonal antibody (84500-1-RR)

    IHC staining of human tonsillitis using 84500-1-RR (same clone as 84500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84500-1-RR (SELPLG/CD162 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84500-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using SELPLG/CD162 Recombinant monoclonal antibody (84500-1-RR)

    IF Staining of Jurkat using 84500-1-RR (same clone as 84500-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SELPLG antibody (84500-1-RR, Clone: 241297G10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84500-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84500-1-RR

    Affinity and Kinetic Characterization of 84500-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84500-1-RR against Human SELPLG/CD162 were performed. The affinity constant is 0.221 nM.

Geprüfte Anwendungen

Produktinformation

84500-1-PBS bindet in IHC, IF/ICC, Indirect ELISA SELPLG/CD162 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name selectin P ligand
Berechnetes Molekulargewicht 412 aa, 43 kDa
GenBank-ZugangsnummerBC029782
Gene symbol SELPLG
Gene ID (NCBI) 6404
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a 120 kDa type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982).