SEMA3A Polyklonaler Antikörper

SEMA3A Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 27836-1-AP

Synonyme

coll 1, Hsema I, Hsema III, Sema III, SEMA1, SEMA3A, SEMAD, SEMAIII, SEMAL, Semaphorin 3A, Semaphorin III, SemD

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  WB analysis using 27836-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27836-1-AP (SEMA3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 27836-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEMA3A antibody (27836-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

27836-1-AP bindet in WB, IF/ICC, CoIP, ELISA SEMA3A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEMA3A fusion protein Ag27173
• Vollständiger Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
• Berechnetes Molekulargewicht: 771 aa, 89 kDa
• Beobachtetes Molekulargewicht: 89 and 65 kDa
• GenBank-Zugangsnummer: BC111416
• Gene symbol: SEMA3A
• Gene ID (NCBI): 10371
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SEMA3A (Semaphorin 3A), a class-3 semaphorin signaling protein, is involved in cell signaling with PlexinA1 and Neuropilin-1 (NRP1) receptors and it is responsible for recruiting dendritic cells into lymphatics. It's widely expressed in different tissues (PMID:10766232). SEMA3A has been shown to exert osteoprotective effects, characterized by increasing bone formation while inhibiting the activation of osteoclast precursor cells (PMID:36598593). However, SEMA3A also modulates the immune system. SEMA3A expression in a myocardial infarct area enhances macrophage polarization and inhibits monocyte migration into the lesion area (PMID:28540528).

Publikationen

Species	Application	Title
human	WB	J Periodontal Res; Semaphorin 3A attenuates the hypoxia suppression of osteogenesis in periodontal ligament stem cells.; Authors - Xiaochi Chang
human	WB	J Orthop Surg Res; Exosomes promote hFOB1.19 proliferation and differentiation via LINC00520; Authors - Jin Wu
rat	WB	Neurosci Lett; TsMS combined with EA promotes functional recovery and axonal regeneration via mediating the miR-539-5p/Sema3A/PlexinA1 signalling axis in sciatic nerve-injured rats; Authors - Xianbin Wang
mouse	WB,IF,CoIP	Sci Adv; Selective promotion of sensory innervation-mediated immunoregulation for tissue repair; Authors - Kaicheng Xu
human	WB	Cell Biochem Biophys; Sema3A Modified PDLSCs Exhibited Enhanced Osteogenic Capabilities and Stimulated Differentiation of Pre-Osteoblasts; Authors - Wen Wang