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SEMA3A Polyklonaler Antikörper

SEMA3A Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27836-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using SEMA3A Polyclonal antibody (27836-1-AP)

    WB analysis using 27836-1-AP (same clone as 27836-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27836-1-AP (SEMA3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27836-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SEMA3A Polyclonal antibody (27836-1-AP)

    IF Staining of HeLa using 27836-1-AP (same clone as 27836-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEMA3A antibody (27836-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 27836-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

27836-1-PBS bindet in WB, IF/ICC, Indirect ELISA SEMA3A und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SEMA3A fusion protein Ag27173
Vollständiger Name sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Berechnetes Molekulargewicht 771 aa, 89 kDa
Beobachtetes Molekulargewicht 89 and 65 kDa
GenBank-ZugangsnummerBC111416
Gene symbol SEMA3A
Gene ID (NCBI) 10371
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SEMA3A (Semaphorin 3A), a class-3 semaphorin signaling protein, is involved in cell signaling with PlexinA1 and Neuropilin-1 (NRP1) receptors and it is responsible for recruiting dendritic cells into lymphatics. It's widely expressed in different tissues (PMID:10766232). SEMA3A has been shown to exert osteoprotective effects, characterized by increasing bone formation while inhibiting the activation of osteoclast precursor cells (PMID:36598593). However, SEMA3A also modulates the immune system. SEMA3A expression in a myocardial infarct area enhances macrophage polarization and inhibits monocyte migration into the lesion area (PMID:28540528).