SEMA4A Rekombinanter Antikörper

SEMA4A Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250325B12

Kat-Nr. : 98513-2-PBS

Synonyme

Sema B, Semab, Semaphorin-4A, Semaphorin-B, SemB

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  FC experiment of mouse splenocytes using 98513-2-RR (same clone as 98513-2-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse SEMA4A Rabbit RecAb (98513-2-RR, Clone: 250325B12) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Mouse CD3. Cells were not fixed. This data was developed using the same antibody clone with 98513-2-PBS in a different storage buffer formulation.

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  FC experiment of mouse splenocytes using 98513-2-RR (same clone as 98513-2-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse SEMA4A Rabbit RecAb (98513-2-RR, Clone: 250325B12) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98513-2-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98513-2-RR (same clone as 98513-2-PBS)

  1x10^6 HEK-293T cells or SEMA4A transfected HEK-293T cells were surface stained with 0.25 ug Anti-Mouse Sema4a Rabbit RecAb (98513-2-RR, Clone: 250325B12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98513-2-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98513-2-RR (same clone as 98513-2-PBS)

  1x10^6 HEK-293T cells (left) or SEMA4A transfected HEK-293T cells (right) were surface stained with 0.25 ug Anti-Mouse SEMA4A Rabbit RecAb (98513-2-RR, Clone: 250325B12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98513-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98513-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98513-2-RR against Mouse SEMA4A were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

98513-2-PBS bindet in FC SEMA4A und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SEMA4A fusion protein Eg2680
• Vollständiger Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A
• Berechnetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: NM_001163489.1
• Gene symbol: Sema4a
• Gene ID (NCBI): 20351
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.