SEMA4C Polyklonaler Antikörper

SEMA4C Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28402-1-PBS

Synonyme

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  WB analysis using 28402-1-AP (same clone as 28402-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 28402-1-AP (same clone as 28402-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28402-1-AP (same clone as 28402-1-PBS)

  Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 28402-1-AP (same clone as 28402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 28402-1-AP (same clone as 28402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 28402-1-AP (same clone as 28402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 28402-1-AP (same clone as 28402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28402-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28402-1-PBS bindet in WB, IHC, Indirect ELISA SEMA4C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEMA4C fusion protein Ag29143
• Vollständiger Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C
• Berechnetes Molekulargewicht: 833 aa, 93 kDa
• Beobachtetes Molekulargewicht: 93 kDa
• GenBank-Zugangsnummer: BC109104
• Gene symbol: SEMA4C
• Gene ID (NCBI): 54910
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SEMA4C is a member of transmembrane semaphorin proteins which regulate axonal guidance in the developing nervous system. Furthermore, studies have demonstrated that sema4C was also implicated in biological events outside the nervous system, such as cell migration, tumor development, and terminal myogenic differentiation (PMID: 36010604). SEMA4C is highly expressed in breast, lung, cervical, and liver cancers. SEMA4C is involved in oncogenic signaling and is a potential therapeutic target for invasive breast cancer, hepatocellular carcinoma, glioma, and osteosarcoma (PMID: 33177246).