SEMA4F Polyklonaler Antikörper

SEMA4F Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12474-1-PBS

Synonyme

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• 

  WB analysis of mouse lung using 12474-1-AP (same clone as 12474-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12474-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Y79 using 12474-1-AP (same clone as 12474-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12474-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12474-1-AP (same clone as 12474-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12474-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12474-1-AP (same clone as 12474-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12474-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 12474-1-AP (same clone as 12474-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12474-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 12474-1-AP (same clone as 12474-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12474-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 12474-1-AP (same clone as 12474-1-PBS)

  IP result of anti-SEMA4F (IP:12474-1-AP, 4ug; Detection:12474-1-AP 1:500) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 12474-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12474-1-PBS bindet in WB, IP, Indirect ELISA SEMA4F und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEMA4F fusion protein Ag3082
• Vollständiger Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F
• Berechnetes Molekulargewicht: 615 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa, 84 kDa
• GenBank-Zugangsnummer: BC018361
• Gene symbol: SEMA4F
• Gene ID (NCBI): 10505
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.