SEMA7A Rekombinanter Antikörper

SEMA7A Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241814B12

Kat-Nr. : 98324-3-RR

Synonyme

CD108, CDw108, John-Milton-Hargen human blood group Ag, Sema K1, Sema L

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  FC experiment of human PBMCs using 98324-3-RR

  1x10^6 untreated or PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human SEMA7A Rabbit RecAb (98324-3-RR, Clone: 241814B12) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of human PBMCs using 98324-3-RR

  1x10^6 PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human SEMA7A Rabbit RecAb (98324-3-RR, Clone: 241814B12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human SEMA7A Rabbit RecAb (98324-3-RR, Clone: 241814B12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PHA treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98324-3-RR bindet in FC SEMA7A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SEMA7A fusion protein Eg2005
• Vollständiger Name: semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)
• Berechnetes Molekulargewicht: 75kDa
• GenBank-Zugangsnummer: NM_003612.5
• Gene symbol: SEMA7A
• Gene ID (NCBI): 8482
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

SEMA7A, also named CD108 and SEMAL, belongs to the semaphorin family. SEMA7A is the only membrane-associated glycosylphosphatidylinositol (GPI)-linked semaphorin and plays a role in the regulation of neuronal axon outgrowth. In addition to its role in guiding axon pathfinding during neuronal development, SEMA7A has diverse functions in morphogenesis and immune cell control and regulates T cell responses via the α1β1 integrin receptor.