SENP1 Rekombinanter Antikörper

SENP1 Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240478F3

Kat-Nr. : 83594-1-PBS

Synonyme

SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478F3

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  IHC staining of human intrahepatic cholangiocarcinoma using 83594-1-RR (same clone as 83594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83594-1-RR (SENP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83594-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83594-1-RR (same clone as 83594-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SENP1 antibody (83594-1-RR, Clone: 240478F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83594-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83594-1-RR (same clone as 83594-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SENP1 Recombinant antibody (83594-1-RR, Clone:240478F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83594-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00607-1

  Cytometric bead array standard curve of MP00607-1, SENP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83594-2-PBS. Detection antibody: 83594-1-PBS. Standard: Ag18028. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83594-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83594-1-PBS against Human SENP1 was performed. The affinity constant is 0.706 nM.

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  Affinity and Kinetic Characterization of 83594-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83594-1-RR against Human SENP1 were performed. The affinity constant is 1.91 nM.

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Geprüfte Anwendungen

Produktinformation

83594-1-PBS bindet in IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA SENP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SENP1 fusion protein Ag18028
• Vollständiger Name: SUMO1/sentrin specific peptidase 1
• Berechnetes Molekulargewicht: 644 aa, 73 kDa
• GenBank-Zugangsnummer: BC045639
• Gene symbol: SENP1
• Gene ID (NCBI): 29843
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.