SENP1 Rekombinanter Antikörper

SENP1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240478D3

Kat-Nr. : 83594-4-PBS

Synonyme

SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478D3

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  WB analysis using 83594-4-RR (same clone as 83594-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83594-4-RR (SENP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83594-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 83594-4-RR (same clone as 83594-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83594-4-RR (SENP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83594-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83594-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83594-4-RR against Human SENP1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83594-4-PBS bindet in WB, IHC, ELISA SENP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SENP1 fusion protein Ag18028
• Vollständiger Name: SUMO1/sentrin specific peptidase 1
• Berechnetes Molekulargewicht: 644 aa, 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC045639
• Gene symbol: SENP1
• Gene ID (NCBI): 29843
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.