SENP5-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SENP5-Specific Polyklonal Antikörper für IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 19529-1-AP

Synonyme

SENP5, Sentrin-specific protease 5, Sentrin/SUMO-specific protease SENP5, EC:3.4.22.-

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  WB analysis of mouse liver using 19529-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19529-1-AP (SENP5-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 19529-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19529-1-AP (SENP5-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19529-1-AP

  IP result of anti-SENP5-Specific (IP:19529-1-AP, 4ug; Detection:19529-1-AP 1:300) with HeLa cells lysate 520ug.

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  IHC staining of human cervical cancer using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

19529-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA SENP5-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SUMO1/sentrin specific peptidase 5
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 97 kDa
• GenBank-Zugangsnummer: NM_152699
• Gene symbol: SENP5
• Gene ID (NCBI): 205564
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SENP5, also named as FKSG45, belongs to the peptidase C48 family. SENP5 is a protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. SENP5 has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. It is required for cell division. The antibody is specific to SENP5.

Publikationen

Species	Application	Title
	WB	EMBO Rep; The SUMO protease SENP3 regulates mitochondrial autophagy mediated by Fis1.; Authors - Emily Waters
	WB	Int J Oncol; Transcription factor E2F4 facilitates SUMOylation to promote HCC progression through interaction with LIN9; Authors - Zhenwei Ma
human	WB	J Cell Sci; SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A.; Authors - Bin Yu
human	WB	J Cell Sci; SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A.; Authors - Bin Yu
human,mouse	WB	iScience; Drp1 SUMO/deSUMOylation by Senp5 isoforms influences ER tubulation and mitochondrial dynamics to regulate brain development.; Authors - Seiya Yamada ; KD Validated
human	WB,IHC	Front Biosci (Landmark Ed); SUMO-Specific Peptidase 5 Promotes Oesophageal Squamous Cell Carcinoma Growth through the NF-κB-SLC1A3 Axis; Authors - Chaoxiang Du ; KD Validated