SEPHS1 Polyklonaler Antikörper

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• KD/KO Validated

SEPHS1 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 16635-1-AP

Synonyme

selenophosphate synthetase 1, Selenophosphate synthase 1, Selenium donor protein 1, Selenide, water dikinase 1, SELD

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  WB analysis of HepG2 using 16635-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16635-1-AP (SEPHS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16635-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16635-1-AP (SEPHS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16635-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16635-1-AP (SEPHS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 16635-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 16635-1-AP (SEPHS1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 16635-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 16635-1-AP (SEPHS1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of human placenta using 16635-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using SEPHS1 antibody (16635-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 16635-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using SEPHS1 antibody (16635-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HepG2-Zellen, Mauslebergewebe, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

16635-1-AP bindet in WB, IHC, IF-P, ELISA SEPHS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEPHS1 fusion protein Ag9978
• Vollständiger Name: selenophosphate synthetase 1
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC000941
• Gene symbol: SEPHS1
• Gene ID (NCBI): 22929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Selenophosphate synthetase 1 (SEPHS1) has been implicated in the maintenance of redox homeostasis in chondrocytes and its deficiency plays a causal role in the progression of osteoarthritis (PMID: 36202216). SEPHS1 downregulation in human umbilical vein cells (HUVECs) led to cell death rather than the inhibition of cell proliferation (PMID: 33622411).

Publikationen

Species	Application	Title
	WB	Biomaterials; H2Se-evolving bio-heterojunctions promote cutaneous regeneration in infected wounds by inhibiting excessive cellular senescence; Authors - Fan Yang
human	WB,IHC	Exp Hematol Oncol; SEPHS1 promotes SMAD2/3/4 expression and hepatocellular carcinoma cells invasion.; Authors - Shu Yang ; KD Validated