Septin 11 Polyklonaler Antikörper

Septin 11 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 14672-1-AP

Synonyme

SEPTIN11, SEPT11, Septin-11

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  WB analysis of human brain using 14672-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14672-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14672-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 14672-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 14672-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 14672-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 14672-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14672-1-AP

  IP result of anti-Septin 11 (IP:14672-1-AP, 4ug; Detection:14672-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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  IHC staining of human ovary using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14672-1-AP (Septin 11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14672-1-AP (Septin 11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14672-1-AP (Septin 11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14672-1-AP (Septin 11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 14672-1-AP

  Immunofluorescent analysis of Hela cells, using Septin 11 antibody 14672-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HepG2 using 14672-1-AP

  Immunofluorescent analysis of HepG2 cells, using Septin 11 antibody 14672-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HeLa using 14672-1-AP

  Immunofluorescent analysis of Hela cells, using Septin 11 antibody 14672-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 14672-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Septin 11 (14672-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HeLa-Zellen, HT-1080.Zellen, Maushirngewebe, Maus-Kolongewebe, Maus-Dünndarmgewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Eierstockgewebe, humanes Nierengewebe, humanes Lungengewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

14672-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA Septin 11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Septin 11 fusion protein Ag6342
• Vollständiger Name: septin 11
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC063615
• Gene symbol: Septin 11
• Gene ID (NCBI): 55752
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	RIP	Cancer Res; CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942.; Authors - Chi Hin Wong
mouse	IF	Cytoskeleton (Hoboken); Characterization of septin expression in normal and fibrotic kidneys.; Authors - Katharina Neubauer
human	IF,WB	Cell Rep; An anillin-CIN85-SEPT9 complex promotes intercellular bridge maturation required for successful cytokinesis; Authors - Thomas C Panagiotou
human	WB	Cancers (Basel); Forchlorfenuron-Induced Mitochondrial Respiration Inhibition and Metabolic Shifts in Endometrial Cancer; Authors - Kyukwang Kim
human,mouse	WB,IP,IF,CoIP	Cell Death Dis; Septin11 promotes hepatocellular carcinoma cell motility by activating RhoA to regulate cytoskeleton and cell adhesion; Authors - Lisheng Fu
human,mouse	IF	Nat Commun; Quantitative determination of the spatial distribution of components in single cells with CellDetail; Authors - Tanja Schuster