SEPT4 Rekombinanter Antikörper

SEPT4 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5O8

Kat-Nr. : 82284-1-PBS

Synonyme

hCDCREL-2, hCDCREL2, Cell division control-related protein 2, C17orf47, BRADEION

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  WB analysis using 82284-1-RR (same clone as 82284-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82284-1-RR (SEPT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82284-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82284-1-RR (same clone as 82284-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82284-1-RR (SEPT4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82284-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82284-1-RR (same clone as 82284-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82284-1-RR (SEPT4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82284-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82284-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82284-1-RR against Human SEPT4 were performed. The affinity constant is 1.38 nM.

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Geprüfte Anwendungen

Produktinformation

82284-1-PBS bindet in WB, IHC, Indirect ELISA SEPT4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SEPT4 fusion protein Ag3185
• Vollständiger Name: septin 4
• Berechnetes Molekulargewicht: 459 aa, 53 kDa
• Beobachtetes Molekulargewicht: 38-55 kDa
• GenBank-Zugangsnummer: BC018056
• Gene symbol: SEPT4
• Gene ID (NCBI): 5414
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spetin 4 is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Spetin 4 was widely expressed in brain and heart, also expressed in colorectal cancers and malignant melanomas. This protein has been implicated in diverse cellular functions, including cytokinesis, apoptosis, and tumor suppression(PMID:15737931). There are several isoforms of SEPTIN4, four of which were detected in the mouse brain and mouse cerebellum, 54 kDa, 52 kDa, 48 kDa and 44 kDa respectively(PMID: 23938054; PMID: 26657011).