Septin 5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Septin 5 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 11631-1-AP

Synonyme

CDCREL, CDCREL 1, CDCREL1, H5, Peanut like protein 1, PNUTL1, SEPT5, Septin 5, septin5

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 11631-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11631-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 11631-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11631-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11631-1-AP

  IP Result of anti-Septin 5 (IP:11631-1-AP, 3ug; Detection:11631-1-AP 1:300) with mouse brain tissue lysate 1000ug.

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  IHC staining of human gliomas using 11631-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11631-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, humanes Hirngewebe, K-562-Zellen, LNCaP-Zellen, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

11631-1-AP bindet in WB, IP, IHC, IF, ELISA Septin 5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Septin 5 fusion protein Ag2205
• Vollständiger Name: septin 5
• Berechnetes Molekulargewicht: 369 aa, 43 kDa
• Beobachtetes Molekulargewicht: 40-43 kDa
• GenBank-Zugangsnummer: BC025261
• Gene symbol: SEPT5
• Gene ID (NCBI): 5413
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Septin 5, a septin family member formerly called cell division control related protein 1 (CDCrel), is encoded in the 200 kb region and is abundantly expressed in the developing and adult brains of mice and humans. Although its constitutive deletion does not cause detectible structural or developmental abnormalities in the mouse brain, Septin 5 has been implicated in subtle neuronal and behavioral functions. Septin 5 is expressed in presynaptic terminals and exerts negative regulatory control over neurotransmitter release by binding to SNARE-protein, which has been implicated in neuropsychiatric disorders (PMID: 19240081). Septin 5 has 2 isoforms with the molecular mass of 39 and 43 kDa.

Publikationen

Species	Application	Title
mouse,rat	WB,IF,IP	J Neurosci; Cyclin-dependent kinase 5 phosphorylation of human septin SEPT5 (hCDCrel-1) modulates exocytosis.; Authors - Amin Niranjana D ND
mouse	WB	Neurobiol Dis; S327 phosphorylation of the presynaptic protein SEPTIN5 increases in the early stages of neurofibrillary pathology and alters the functionality of SEPTIN5.; Authors - Catarina B Ferreira
mouse	WB	Front Mol Neurosci; Integrated proteomics and metabolomics analysis of D-pinitol function during hippocampal damage in streptozocin-induced aging-accelerated mice; Authors - Xiaoxia Li
human	IF,WB	Cells; Presynaptic Vesicle Protein SEPTIN5 Regulates the Degradation of APP C-Terminal Fragments and the Levels of Aβ.; Authors - Mikael Marttinen ; KO Validated
rat	WB	Molecules; Synergistic Neuroprotective Effect of Endogenously-Produced Hydroxytyrosol and Synaptic Vesicle Proteins on Pheochromocytoma Cell Line Against Salsolinol.; Authors - Robina Manzoor
mouse	IF	Cytoskeleton (Hoboken); Characterization of septin expression in normal and fibrotic kidneys.; Authors - Katharina Neubauer