Septin 6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Septin 6 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Rezensionen (1)

Kat-Nr. : 12805-1-AP

Synonyme

KIAA0128, SEP2, SEPT2, SEPT6, septin 6, septin6

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 12805-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12805-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 12805-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12805-1-AP (Septin 6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 12805-1-AP

  IP Result of anti-Septin 6 (IP:12805-1-AP, 4ug; Detection:12805-1-AP 1:600) with rat brain tissue lysate 4000ug.

• 

  IHC staining of human cervical cancer using 12805-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12805-1-AP (Septin 6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12805-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12805-1-AP (SEPT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

12805-1-AP bindet in WB, IP, IHC, IF, ELISA Septin 6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Septin 6 fusion protein Ag3521
• Vollständiger Name: septin 6
• Berechnetes Molekulargewicht: 434 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC036240
• Gene symbol: SEPT6
• Gene ID (NCBI): 23157
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.

Publikationen

Species	Application	Title
human	WB	J Biol Chem; Uncovering principles that control septin-septin interactions.; Authors - Moshe S Kim
human,rat	WB	Lab Invest; Effect of SEPT6 on the biological behavior of hepatic stellate cells and liver fibrosis in rats and its mechanism.; Authors - Yuhui Fan ; KD Validated
mouse	WB	Proteomics; Quantification of protein isoforms in mesenchymal stem cells by reductive dimethylation of lysines in intact proteins.; Authors - She Yi-Min YM
human	IHC	Int J Oncol; SEPT6 drives hepatocellular carcinoma cell proliferation, migration and invasion via the Hippo/YAP signaling pathway.; Authors - Yuhui Fan
human	WB	Mol Biol Cell; Revised subunit order of mammalian septin complexes explains their in vitro polymerization properties.; Authors - Forooz Soroor
human	IF	Mol Hum Reprod; The SEPT12 complex is required for the establishment of a functional sperm head-tail junction.; Authors - Yi-Ru Shen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mohammed (Verified Customer) (08-19-2020)

Did not show strong staining at the sperm annulus. Overall very high background.

• Applications: Immunofluorescence
• Primary Antibody Dilution: (1:200)
• Cell Tissue Type: Testis