SERBP1 Polyklonaler Antikörper

SERBP1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 10729-1-AP

Synonyme

PAIRBP1, PAI1 RNA-binding protein 1, PAI RBP1, HABP4L, CHD3IP

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  WB analysis using 10729-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10729-1-AP (SERBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10729-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10729-1-AP (SERBP1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 10729-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10729-1-AP (SERBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10729-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10729-1-AP (SERBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 10729-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using SERBP1 antibody (10729-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

10729-1-AP bindet in WB, IHC, IF/ICC, ELISA SERBP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SERBP1 fusion protein Ag1045
• Vollständiger Name: SERPINE1 mRNA binding protein 1
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC008045
• Gene symbol: SERBP1
• Gene ID (NCBI): 26135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The plasminogen activator system (PA) plays an important role in invasion and metastasis of solid tumors. The PA Inhibitor type 1 (PAI-1) is the main physiologic regulator of plasminogen activation. PAI-RBP1 (PAI-1 mRNA Binding Protein 1, also named SERBP1), is an RNA-binding protein containing an RG-rich domain and an RGG box domain that might be methylated by protein arginine N-methyltransferases (PRMTs). SERBP1 was identified as an interacting partner of a Tudor domain containing protein TDRD3, and can localize to cytoplasmic stress granules (SGs).

Publikationen

Species	Application	Title
		Cell Rep; Triggering endogenous Z-RNA sensing for anti-tumor therapy through ZBP1-dependent necroptosis; Authors - Tao Yang
human	WB	J Clin Endocrinol Metab; The HMGA2-IMP2 Pathway Promotes Granulosa Cell Proliferation in Polycystic Ovary Syndrome.; Authors - Miao Li
human	WB	Front Genet; Identification of immune signatures in Parkinson's disease based on co-expression networks; Authors - Xiaolin Dong