SERCA2/ATP2A2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SERCA2/ATP2A2 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Rezensionen (1)

Kat-Nr. : 27311-1-AP

Synonyme

ATP2A2, SERCA2, SERCA2,ATP2A2, SERCA2b, ATP2A 2

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  WB analysis using 27311-1-AP

  Boiled and unboiled mouse heart lysates were subjected to SDS PAGE followed by western blot with 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27311-1-AP

  Boiled and 37°C incubated HEK-293 cells lysates were subjected to SDS PAGE followed by western blot with 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 27311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 27311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 27311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 27311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 27311-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 27311-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 27311-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 27311-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse heart tissue using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of C2C12 using 27311-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausherzgewebe
• Erfolgreiche Detektion in IF-Fro: Mausherzgewebe
• Erfolgreiche Detektion in IF/ICC: C2C12-Zellen, Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

27311-1-AP bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA SERCA2/ATP2A2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SERCA2/ATP2A2 fusion protein Ag26177
• Vollständiger Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• Berechnetes Molekulargewicht: 1042 aa, 115 kDa
• Beobachtetes Molekulargewicht: 110-115 kDa
• GenBank-Zugangsnummer: BC035588
• Gene symbol: SERCA2
• Gene ID (NCBI): 488
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.

Publikationen

Species	Application	Title
mouse	WB	Life Sci; Trimethylamine N-oxide induces cardiac diastolic dysfunction by down-regulating Piezo1 in mice with heart failure with preserved ejection fraction; Authors - Qian Chen
mouse	IF	Am J Physiol Lung Cell Mol Physiol; Role of voltage-gated Ca2+ channels and Ano1 Ca2+-activated Cl- channels in M2 muscarinic receptor-dependent contractions of murine airway smooth muscle; Authors - Srijit Ghosh
mouse	WB	JCI Insight; Critical role of Znhit1 for postnatal heart function and vacuolar cardiomyopathy.; Authors - Yingchao Shi
human	WB	Nat Commun; ER calcium depletion as a key driver for impaired ER-to-mitochondria calcium transfer and mitochondrial dysfunction in Wolfram syndrome; Authors - Mailis Liiv
human	WB	Biochem Biophys Res Commun; Mitigation of ROS-triggered endoplasmic reticulum stress by upregulating Nrf2 retards diabetic nephropathy; Authors - Xiaojiao Zeng
human	WB,IHC	FASEB J; Pilocarpine mediated excessive calcium accumulation leads to ciliary muscle cell senescence and apoptosis; Authors - Xiang Gao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Paulina (Verified Customer) (03-26-2025)

Fixation: 2% PFA for 20min. Permeabilization/antibody dilution solution: 0.05% saponin, 5% horse serum in PBS. ATP1A1 antibody diluted in saponin solution & incubated for 1 hour at room temperature, followed by 2ary anti-rabbit conjugated to AlexaFluor 488 (1:200 in saponin solution) for 1 hour. Signal shows correct localization at ER, although it is not super strong, but this could be due to low expression in this cell line.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: MDA-MB-231 cells

SERCA2/ATP2A2 Antibody Immunofluorescence validation (1:100 dilution) in MDA-MB-231 cells (Cat no:27311-1-AP)