SERCA2,ATP2A2 Monoklonaler Antikörper

SERCA2,ATP2A2 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F11A11

Kat-Nr. : 67248-1-PBS

Synonyme

ATP2A2, SERCA2, SERCA2b, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, Calcium pump 2

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  WB analysis of HEK-293 using 67248-1-Ig (same clone as 67248-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67248-1-Ig (same clone as 67248-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67248-1-Ig (same clone as 67248-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67248-1-Ig (same clone as 67248-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67248-1-Ig (same clone as 67248-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 67248-1-Ig (same clone as 67248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 67248-1-Ig (same clone as 67248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67248-1-Ig (same clone as 67248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67248-1-Ig (same clone as 67248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67248-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67248-1-PBS bindet in WB, IHC, Indirect ELISA SERCA2,ATP2A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SERCA2,ATP2A2 fusion protein Ag5052
• Vollständiger Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• Berechnetes Molekulargewicht: 1042 aa, 115 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC035588
• Gene symbol: SERCA2
• Gene ID (NCBI): 488
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.