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SERCA2/ATP2A2 Rekombinanter Antikörper

SERCA2/ATP2A2 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250667D7

Kat-Nr. : 86050-1-PBS

Synonyme

ATP2A2, SERCA2, SERCA2b, ATP2A 2, ATP2B

Filter:
  • Western Blot (WB) analysis of various lysates using SERCA2/ATP2A2 Recombinant antibody (86050-1-RR)

    WB analysis using 86050-1-RR (same clone as 86050-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86050-1-RR (SERCA2,ATP2A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SERCA2/ATP2A2 Recombinant antibody (86050-1-RR)

    IHC staining of mouse skeletal muscle using 86050-1-RR (same clone as 86050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 86050-1-RR (SERCA2/ATP2A2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86050-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using SERCA2/ATP2A2 Recombinant antibody (86050-1-RR)

    IF Staining of C2C12 using 86050-1-RR (same clone as 86050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using SERCA2/ATP2A2 antibody (86050-1-RR, Clone: 250667D7 ) at dilution of 1:0 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86050-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86050-1-RR

    Affinity and Kinetic Characterization of 86050-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86050-1-RR against Human SERCA2/ATP2A2 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

86050-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SERCA2/ATP2A2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen SERCA2/ATP2A2 fusion protein Ag26177
Vollständiger Name ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Berechnetes Molekulargewicht 1042 aa, 115 kDa
Beobachtetes Molekulargewicht110-115 kDa
GenBank-ZugangsnummerBC035588
Gene symbol SERCA2
Gene ID (NCBI) 488
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.