SERP1 Polyklonaler Antikörper

SERP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17807-1-PBS

Synonyme

RAMP4, Ribosome-attached membrane protein 4, Stress-associated endoplasmic reticulum protein 1

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  WB analysis of mouse brain using 17807-1-AP (same clone as 17807-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17807-1-AP (SERP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 17807-1-AP (same clone as 17807-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17807-1-AP (SERP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse pancreas using 17807-1-AP (same clone as 17807-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 17807-1-AP (SERP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 17807-1-AP (same clone as 17807-1-PBS)

  IP result of anti-SERP1 (IP:17807-1-AP, 4ug; Detection:17807-1-AP 1:500) with mouse brain tissue lysate 3160 ug. This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 17807-1-AP (same clone as 17807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 17807-1-AP (SERP1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 17807-1-AP (same clone as 17807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 17807-1-AP (SERP1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 17807-1-AP (same clone as 17807-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SERP1 antibody (17807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17807-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA SERP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SERP1 fusion protein Ag12087
• Vollständiger Name: stress-associated endoplasmic reticulum protein 1
• Berechnetes Molekulargewicht: 66 aa, 7 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC108314
• Gene symbol: SERP1
• Gene ID (NCBI): 27230
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stress-associated endoplasmic reticulum (ER) protein 1 (SERP1), also known as ribosome- associated membrane protein 4 (RAMP4), is a Sec61-associated polypeptide that is induced by ER stress [PMID:16705175]. SERP1 interacts with target proteins during their translocation into the lumen of the endoplasmic reticulum. It controls glycosylation of major histocompatibility complex class II-associated invariant chains by a translocational pausing mechanism, and its overexpression stabilizes newly synthesized membrane proteins under ER stress by associating with the Sec61 complex [PMID:10601334]. It is suggested SERP1 is involved in the biosynthesis/processing of secretory proteins