Alpha Antichymotrypsin Polyklonaler Antikörper

Alpha Antichymotrypsin Polyklonal Antikörper für FC, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 12192-1-AP

Synonyme

A1 Antichymotrypsin, A-1-Antichymotrypsin, AACT, ACT, Alpha 1 antichymotrypsin, GIG24, GIG25, Serpin A3, SERPINA3

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Galerie der Validierungsdaten

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  WB analysis of human plasma using 12192-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 12192-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12192-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 12192-1-AP

  IP Result of anti-alpha 1 Antichymotrypsin (IP:12192-1-AP, 4ug; Detection:12192-1-AP 1:500) with COLO 320 cells lysate 2000ug.

• 

  IHC staining of human tonsillitis using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HepG2 using 12192-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Alpha Antichymotrypsin (12192-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Blutgewebe, COLO 320-Zellen, humanes Kolongewebe
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lebergewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

12192-1-AP bindet in WB, IP, IHC, FC, ELISA Alpha Antichymotrypsin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Alpha Antichymotrypsin fusion protein Ag2830
• Vollständiger Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
• Berechnetes Molekulargewicht: 448 aa, 48 kDa
• Beobachtetes Molekulargewicht: 44-70 kDa
• GenBank-Zugangsnummer: BC010530
• Gene symbol: SERPINA3
• Gene ID (NCBI): 12
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.

Publikationen

Species	Application	Title
		Acta Neuropathol; Integrative proteomics highlight presynaptic alterations and c-Jun misactivation as convergent pathomechanisms in ALS; Authors - Amr Aly
human	WB	Front Immunol; Screening and identification of tissue-infiltrating immune cells and genes for patients with emphysema phenotype of COPD; Authors - Di Wang
human	WB,IHC	Br J Cancer; Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.; Authors - Yanxia Jin
mouse	IHC	Front Med (Lausanne); An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury.; Authors - Songyan Gao
		Proteomics Clin Appl; Serum protein profiling in diffuse large B-cell lymphoma.; Authors - Jacques Riby
human,rat	WB	Food Funct; Sinensetin attenuates IL-1β-induced cartilage damage and ameliorates osteoarthritis by regulating SERPINA3; Authors - Zhendong Liu