Alpha Antichymotrypsin Monoklonaler Antikörper

Alpha Antichymotrypsin Monoklonal Antikörper für FC, IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

3F2F10

Kat-Nr. : 66078-1-Ig

Synonyme

A1 Antichymotrypsin, A-1-Antichymotrypsin, AACT, ACT, Alpha 1 antichymotrypsin, GIG24, GIG25, Serpin A3, SERPINA3

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Galerie der Validierungsdaten

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  WB analysis of human plasma using 66078-1-Ig

  Untreated and PNGase F-treated lysates of human plasma were subjected to SDS PAGE followed by western blot with 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. Loading amount：0.7 μL crude plasma was lysised for each lane. PNGase F was obtained from Atagenix (cat.NO. ata808).

• 

  WB analysis of human colon using 66078-1-Ig

  Untreated and PNGase F-treated lysates of human colon tissue (25μg/lane) were subjected to SDS PAGE followed by western blot with 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

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  IHC staining of human tonsillitis using 66078-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66078-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 66078-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66078-1-Ig(alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 66078-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66078-1-Ig(alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human tonsillitis using 66078-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 66078-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Alpha Antichymotrypsin (66078-1-Ig, Clone:3F2F10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma, humanes Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

66078-1-Ig bindet in WB, IHC, IF, FC, ELISA Alpha Antichymotrypsin und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Alpha Antichymotrypsin fusion protein Ag2830
• Vollständiger Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
• Berechnetes Molekulargewicht: 448 aa, 48 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC010530
• Gene symbol: SERPINA3
• Gene ID (NCBI): 12
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.

Publikationen

Species	Application	Title
		Br J Cancer; Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.; Authors - Yanxia Jin
human	WB,IHC	Breast Cancer; Overexpression of SERPINA3 promotes tumor invasion and migration, epithelial-mesenchymal-transition in triple-negative breast cancer cells.; Authors - Yingzi Zhang
human	IHC	Pharmaceutics; Distribution of Monocarboxylate Transporters in Brain and Choroid Plexus Epithelium; Authors - Masaki Ueno