Serpin A4/Kallistatin Polyklonaler Antikörper

Serpin A4/Kallistatin Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 11430-2-AP

Synonyme

Kallistatin, Serpin A4, KAL, Kallikrein inhibitor, KLST

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  WB analysis of human spleen using 11430-2-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11430-2-AP (Kallistatin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 11430-2-AP

  human serum were subjected to SDS PAGE followed by western blot with 11430-2-AP (Kallistatin antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11430-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11430-2-AP (Kallistatin antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HCT 116 using 11430-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using Kallistatin antibody (11430-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Milzgewebe, humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HCT 116-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

11430-2-AP bindet in WB, IHC, IF/ICC, ELISA Serpin A4/Kallistatin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Serpin A4/Kallistatin fusion protein Ag1962
• Vollständiger Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC014992
• Gene symbol: Kallistatin
• Gene ID (NCBI): 5267
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kallistatin is also named Serpin A4 and Peptidase inhibitor 4 (PI-4). Kallistatin was first identified in human plasma as a tissue kallikrein-binding protein and a serine proteinase inhibitor. Kallistatin via its two structural elements regulates differential signaling cascades, and thus a wide spectrum of biological functions (PMID: 28427524). Kallistatin via the active site promotes neovascularization by stimulating VEGF levels in endothelial progenitor cells. Second, kallistatin inhibits or induces apoptosis depending on cell types. Kallistatin via its heparin-binding site inhibits angiogenesis by blocking vascular endothelial growth factor (VEGF)-induced growth, migration and adhesion of endothelial cells. Kallistatin can act as an antioxidant or pro-oxidant (PMID: 28742513).

Publikationen

Species	Application	Title
mouse	WB,IF	Diabetes; Pathogenic Role of Diabetes-induced Overexpression of Kallistatin in Corneal Wound Healing Deficiency through Inhibition of Canonical Wnt Signaling.; Authors - Wentao Liang