SERPINA7/ TBG Monoklonaler Antikörper

SERPINA7/ TBG Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1B11B10

Kat-Nr. : 66454-1-Ig

Synonyme

SERPINA7, 1B11B10, Serpin A7, SERPINA 7

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  WB analysis of human plasma using 66454-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66454-1-Ig (SERPINA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 66454-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66454-1-Ig (SERPINA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66454-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66454-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66454-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66454-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66454-1-Ig (SERPINA7 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 66454-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERPINA7 antibody (66454-1-Ig, Clone: 1B11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 66454-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERPINA7 antibody (66454-1-Ig, Clone: 1B11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe, humanes Hodengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Leberkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

66454-1-Ig bindet in WB, IHC, IF-P, ELISA SERPINA7/ TBG und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SERPINA7/ TBG fusion protein Ag9823
• Vollständiger Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
• Berechnetes Molekulargewicht: 415 aa, 46 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC020747
• Gene symbol: SERPINA7
• Gene ID (NCBI): 6906
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Heliyon; Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study; Authors - Lingyan Chen