SERPINB13 Polyklonaler Antikörper

SERPINB13 Polyklonal Antikörper für IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18045-1-AP

Synonyme

Serpin B13, HaCaT UV repressible serpin, HaCaT UV-repressible serpin, headpin, HUR7

---

• 

  IHC staining of human oesophagus cancer using 18045-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18045-1-AP (SERPINB13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 18045-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18045-1-AP (SERPINB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 18045-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18045-1-AP (SERPINB13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 18045-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18045-1-AP (SERPINB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 18045-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18045-1-AP (SERPINB13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 18045-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18045-1-AP (SERPINB13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 18045-1-AP

  Immunofluorescent analysis of HepG2 cells, using SERPINB13 antibody 18045-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 18045-1-AP

  mmunofluorescent analysis of Hela cells, using SERPINB13 antibody 18045-1-AP at 1:25 dilution and FITC-Donkey anti-Rabbit IgG (green).

• 

  IF Staining of HeLa using 18045-1-AP

  Immunofluorescent analysis of Hela cells, using SERPINB13 antibody 18045-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 18045-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug SERPINB13 Polyclonal antibody (18045-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe, humanes Hautgewebe, Maushautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18045-1-AP bindet in IHC, IF/ICC, FC (Intra), ELISA SERPINB13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SERPINB13 fusion protein Ag12686
• Vollständiger Name: serpin peptidase inhibitor, clade B (ovalbumin), member 13
• Berechnetes Molekulargewicht: 391 aa, 44 kDa
• GenBank-Zugangsnummer: BC101821
• Gene symbol: SERPINB13
• Gene ID (NCBI): 5275
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.