SERPINB9 Monoklonaler Antikörper

SERPINB9 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2H9D2

Kat-Nr. : 67950-1-Ig

Synonyme

CAP 3, CAP3, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, PI 9, PI9, Serpin B9, SERPINB9

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  WB analysis of K-562 using 67950-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 67950-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 67950-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Ramos using 67950-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67950-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 67950-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67950-1-Ig (SERPINB9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 67950-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67950-1-Ig (SERPINB9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C6 using 67950-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using SERPINB9 antibody (67950-1-Ig, Clone: 2H9D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Daudi-Zellen, humanes Plazenta-Gewebe, Raji-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: C6-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

67950-1-Ig bindet in WB, IHC, IF/ICC, ELISA SERPINB9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SERPINB9 fusion protein Ag6719
• Vollständiger Name: serpin peptidase inhibitor, clade B (ovalbumin), member 9
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC002538
• Gene symbol: SERPINB9
• Gene ID (NCBI): 5272
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINB9, also named as PI-9, belongs to the serpin family. SERPINB9 is an intracellular inhibitor of granzyme B (grB) that protects cytotoxic lymphocytes from grB-mediated death. It has a nucleo-cytoplasmic distribution and is produced in CD8+ T cells and natural killer cells (PMID: 28024184). Also, SERPINB9 is expressed by vascular SMCs and endothelial cells to avoid GZB-induced apoptosis (PMID: 28743062). The molecular weight of SERPINB9 is 42 kDa.

Publikationen

Species	Application	Title
mouse	WB	Immunotherapy; The development of anti-PD-1 antibody-induced spinal cord injury in bone marrow transplant C57BL/6 Rag1-/- mouse model; Authors - Huachun Chen
human	IHC,IF	Cell Death Dis; Single-cell landscape identified SERPINB9 as a key player contributing to stemness and metastasis in non-seminomas; Authors - Zhouliang Bian ; KD Validated