SERPINB9 Monoklonaler Antikörper

SERPINB9 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H9D2

Kat-Nr. : 67950-1-PBS

Synonyme

CAP 3, CAP3, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, PI 9, PI9, Serpin B9, SERPINB9

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  WB analysis of K-562 using 67950-1-Ig (same clone as 67950-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 67950-1-Ig (same clone as 67950-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 67950-1-Ig (same clone as 67950-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 67950-1-Ig (same clone as 67950-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67950-1-Ig (same clone as 67950-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 67950-1-Ig (same clone as 67950-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67950-1-Ig (SERPINB9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 67950-1-Ig (same clone as 67950-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67950-1-Ig (SERPINB9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

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  IF Staining of C6 using 67950-1-Ig (same clone as 67950-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using SERPINB9 antibody (67950-1-Ig, Clone: 2H9D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67950-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SERPINB9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SERPINB9 fusion protein Ag6719
• Vollständiger Name: serpin peptidase inhibitor, clade B (ovalbumin), member 9
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC002538
• Gene symbol: SERPINB9
• Gene ID (NCBI): 5272
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINB9, also named as PI-9, belongs to the serpin family. SERPINB9 is an intracellular inhibitor of granzyme B (grB) that protects cytotoxic lymphocytes from grB-mediated death. It has a nucleo-cytoplasmic distribution and is produced in CD8+ T cells and natural killer cells (PMID: 28024184). Also, SERPINB9 is expressed by vascular SMCs and endothelial cells to avoid GZB-induced apoptosis (PMID: 28743062). The molecular weight of SERPINB9 is 42 kDa.