SERPINE2 Rekombinanter Antikörper

SERPINE2 Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5O16

Kat-Nr. : 82732-5-PBS

Synonyme

PI7, PN1, Serpin E2, 5O16, GDN

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  WB analysis using 82732-5-RR (same clone as 82732-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82732-5-RR (SERPINE2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82732-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 82732-5-RR (same clone as 82732-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82732-5-RR (SERPINE2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82732-5-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 82732-5-RR (same clone as 82732-5-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug SERPINE2 Recombinant antibody (82732-5-RR, Clone:5O16) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82732-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82732-5-RR

  Biolayer interferometry (BLl) kinetic assays of 82732-5-RR against Human SERPINE2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82732-5-PBS bindet in WB, FC (Intra), Indirect ELISA SERPINE2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SERPINE2 fusion protein Ag1830
• Vollständiger Name: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44-50 kDa
• GenBank-Zugangsnummer: BC015663
• Gene symbol: SERPINE2
• Gene ID (NCBI): 5270
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.