Alpha 2-Antiplasmin Polyklonaler Antikörper

Alpha 2-Antiplasmin Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 13228-1-AP

Synonyme

A2AP, AAP, Alpha 2 antiplasmin, Alpha 2 AP, ALPHA 2 PI, Alpha 2 plasmin inhibitor, Alpha-2-antiplasmin, API, PLI, Serpin F2, SERPINF2

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 13228-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13228-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13228-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13228-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 13228-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Lebergewebe, K-562-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

13228-1-AP bindet in WB, IF, ELISA Alpha 2-Antiplasmin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Alpha 2-Antiplasmin fusion protein Ag4095
• Vollständiger Name: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
• Berechnetes Molekulargewicht: 491 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC031592
• Gene symbol: SERPINF2
• Gene ID (NCBI): 5345
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Dis Markers; Identification of differentially expressed serum proteins in infectious purpura fulminans.; Authors - Ting He
human	WB	Res Pract Thromb Haemost; Citrullination of α2-antiplasmin is unlikely to contribute to enhanced plasmin generation in COVID-19 pathophysiology; Authors - Kadri Kangro