SESN1 Rekombinanter Antikörper

SESN1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250470G1

Kat-Nr. : 86008-1-RR

Synonyme

EC:1.11.1.-, p53 regulated protein PA26, p53-regulated protein PA26, PA26, SEST1

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  WB analysis using 86008-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86008-1-RR (SESN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86008-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86008-1-RR against Human SESN1 were performed. The affinity constant is 1.01 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Skelettmuskelgewebe, humanes Hodengewebe, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:30000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86008-1-RR bindet in WB, ELISA SESN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SESN1 fusion protein Ag16462
• Vollständiger Name: sestrin 1
• Berechnetes Molekulargewicht: 551 aa, 64 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC112036
• Gene symbol: SESN1
• Gene ID (NCBI): 27244
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 1 is implicated in the inhibition of cell growth. It is approximately a 66kDa protein in humans (551 amino acids).