Sestrin 2 Polyklonaler Antikörper

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• KD/KO Validated

Sestrin 2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 21346-1-AP

Synonyme

SESN2, Sestrin2, SESN 2, SES2, Hypoxia-induced gene

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  WB analysis of mouse thymus using 21346-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 21346-1-AP (Sestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 21346-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21346-1-AP (Sestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21346-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21346-1-AP (Sestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 21346-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21346-1-AP (Sestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21346-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21346-1-AP (Sestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U2OS using 21346-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Sestrin 2 antibody (21346-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe, HeLa-Zellen, K-562-Zellen, Mauslungengewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IF: See 5 publications below

Produktinformation

21346-1-AP bindet in WB, IF/ICC, ELISA Sestrin 2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Sestrin 2 fusion protein Ag15669
• Vollständiger Name: sestrin 2
• Berechnetes Molekulargewicht: 480 aa, 54 kDa
• Beobachtetes Molekulargewicht: 60-66 kDa
• GenBank-Zugangsnummer: NM_031459
• Gene symbol: SESN2/Sestrin 2
• Gene ID (NCBI): 83667
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell growth and survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).

Publikationen

Species	Application	Title
human	WB	Cancer Cell; p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.; Authors - Alessandro Carugo
human	WB	Elife; Dynamics of mTORC1 activation in response to amino acids.; Authors - Maria Manifava ; KD Validated
mouse	WB,IF	Front Immunol; Sestrin2 provides cerebral protection through activation of Nrf2 signaling in microglia following subarachnoid hemorrhage; Authors - Youqing Yang
human	WB,IF	J Invest Dermatol; Metastatic melanoma cells rely on Sestrin2 to acquire anoikis resistance via detoxifying intracellular ROS.; Authors - Guannan Zhu ; KD Validated
rat	WB	Mol Cell Biol; Sestrin2 protects dopaminergic cells against rotenone toxicity through AMPK-dependent autophagy activation.; Authors - Yi-Sheng Hou
rat	IF	J Cereb Blood Flow Metab; Sestrin2, as a negative feedback regulator of mTOR, provides neuroprotection by activation AMPK phosphorylation in neonatal hypoxic-ischemic encephalopathy in rat pups.; Authors - Xudan Shi