SESN3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SESN3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 24532-1-AP

Synonyme

SESN3, SEST3, sestrin 3

---

Galerie der Validierungsdaten

• 

  WB analysis of A431 using 24532-1-AP

  WB result of SESN3 antibody (24532-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SESN3 transfected A431 cells.

• 

  WB analysis of HepG2 using 24532-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24532-1-AP (SESN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

24532-1-AP bindet in WB, IHC, ELISA SESN3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SESN3 fusion protein Ag21380
• Vollständiger Name: sestrin 3
• Berechnetes Molekulargewicht: 492 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC017296
• Gene symbol: SESN3
• Gene ID (NCBI): 143686
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT induced increase in ROS and it might be a promising target in selectively killing cancer cells containing high levels of AKT activity.

Publikationen

Species	Application	Title
human	IHC	J Orthop Res; Age-related reduction in the expression of FOXO transcription factors and correlations with intervertebral disc degeneration.; Authors - Oscar Alvarez-Garcia
human	WB	Mol Cell; Ring domains are essential for GATOR2-dependent mTORC1 activation; Authors - Cong Jiang