SESN3 Rekombinanter Antikörper

SESN3 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242189D1

Kat-Nr. : 84823-3-PBS

Synonyme

242189D1, EC:1.11.1.-, SEST3, sestrin 3, Sestrin-3

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  WB analysis using 84823-3-RR (same clone as 84823-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84823-3-RR (SESN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84823-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84823-3-RR (same clone as 84823-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84823-3-RR (SESN3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84823-3-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84823-3-RR (same clone as 84823-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SESN3 antibody (84823-3-RR, Clone: 242189D1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84823-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84823-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84823-3-RR against Human SESN3 were performed. The affinity constant is 6.78 nM.

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Geprüfte Anwendungen

Produktinformation

84823-3-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SESN3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SESN3 fusion protein Ag17872
• Vollständiger Name: sestrin 3
• Berechnetes Molekulargewicht: 492 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57-60 kDa
• GenBank-Zugangsnummer: BC017296
• Gene symbol: SESN3
• Gene ID (NCBI): 143686
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT-induced increase in ROS. It might be a promising target in selectively killing cancer cells with high AKT activity levels.