SET/TAF-I Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SET/TAF-I Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 55201-1-AP

Synonyme

2PP2A, HLA DR associated protein II, I 2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, Protein SET, SET, SET nuclear oncogene, SET/TAF-I, TAF I, TAF IBETA, Template activating factor I

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Galerie der Validierungsdaten

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  WB analysis using 55201-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55201-1-AP (SET/TAF-I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 55201-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55201-1-AP (SET/TAF-I antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 55201-1-AP (SET/TAF-I antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 55201-1-AP (SET/TAF-I antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55201-1-AP (SET Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55201-1-AP (SET Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55201-1-AP (SET antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55201-1-AP (SET antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55201-1-AP (SET antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55201-1-AP (SET antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Mausmilzgewebe, humanes Gliomgewebe, humanes Leberkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below

Produktinformation

55201-1-AP bindet in WB, IHC, ELISA SET/TAF-I und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SET nuclear oncogene
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 41 kDa, 39 kDa
• GenBank-Zugangsnummer: NM_003011
• Gene symbol: SET
• Gene ID (NCBI): 6418
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SET, also named as IGAAD, I-2PP2A, TAF-I, IGAAD and PHAPII, belongs to the nucleosome assembly protein (NAP) family.SET is a multifunctional oncoprotein which is ubiquitously expressed in all kinds of cells. The SET protein participates in many cellular processes including cell cycle, cell migration, apoptosis, transcription, and DNA repair. The SET protein plays important roles in facilitating cell growth and proliferation via many pathways that promote tumorigenesis and metastasis. The SET protein shows apparent molecular masses of 41 and 39 kDa (PMID: 12759364, PMID: 7753797).

Publikationen

Species	Application	Title
human	WB	Cell Rep; A p53/LINC00324 positive feedback loop suppresses tumor growth by counteracting SET-mediated transcriptional repression; Authors - Ling Zhang ; KO Validated
human	WB	Oncotarget; Overexpression of PP2A inhibitor SET oncoprotein is associated with tumor progression and poor prognosis in human non-small cell lung cancer.; Authors - Hao Liu ; KD Validated
human	WB	J Med Chem; Selective Peptidomimetic Inhibitors of NTMT1/2: Rational Design, Synthesis, Characterization, and Crystallographic Studies.; Authors - Brianna D Mackie
human	WB	Cancers (Basel); Pre-Clinical Study Evaluating Novel Protein Phosphatase 2A Activators as Therapeutics for Neuroblastoma.; Authors - Laura V Bownes
human	WB	Cell Signal; PMID: 25152373; Authors - Hao Liu
human	WB,IHC	BMC Cancer; MicroRNA-1915-3p inhibits cell migration and invasion by targeting SET in non-small-cell lung cancer.; Authors - Hongli Pan