SET Monoklonaler Antikörper

SET Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

2B4D1

Rezensionen (1)

Kat-Nr. : 67478-1-Ig

Synonyme

2PP2A, HLA DR associated protein II, I 2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, Protein SET, SET, SET nuclear oncogene, TAF I, TAF IBETA, Template activating factor I

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 67478-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67478-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 67478-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67478-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67478-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, 4T1-Zellen, HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67478-1-Ig bindet in WB, ELISA SET und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SET nuclear oncogene
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33-36 kDa
• GenBank-Zugangsnummer: NM_003011
• Gene symbol: SET
• Gene ID (NCBI): 6418
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SET, also named as IGAAD, I-2PP2A, TAF-I, IGAAD and PHAPII, belongs to the nucleosome assembly protein (NAP) family.SET is a multifunctional oncoprotein which is ubiquitously expressed in all kinds of cells. The SET protein participates in many cellular processes including cell cycle, cell migration, apoptosis, transcription, and DNA repair. The SET protein plays important roles in facilitating cell proliferation via many pathways that promote tumorigenesis and metastasis.

Publikationen

Species	Application	Title
human	WB	Cell Rep; A p53/LINC00324 positive feedback loop suppresses tumor growth by counteracting SET-mediated transcriptional repression; Authors - Ling Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chantal (Verified Customer) (10-04-2022)

Specific band appeared after 3 minutes of exposure. it works on mouse and human cell lines

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MEFs, Hela, HEK293FT

SET Antibody Western Blot validation (1:1000 dilution) in MEFs, Hela, HEK293FT (Cat no:67478-1-Ig)