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SETD1B Polyklonaler Antikörper

SETD1B Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 55005-1-AP

Synonyme

EC:2.1.1.364, Histone-lysine N-methyltransferase SETD1B, hSET1B, KIAA1076, KMT2G



Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Hirngewebe, MCF-7-Zellen, Mauslebergewebe
Erfolgreiche IPMCF-7-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Ovarialkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

55005-1-AP bindet in WB, IHC, IF/ICC, IP, ChIP, ELISA SETD1B und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name SET domain containing 1B
Berechnetes Molekulargewicht 209 kDa
Beobachtetes Molekulargewicht 250-260 kDa, 450 kDa
GenBank-ZugangsnummerNM_015048
Gene symbol SETD1B
Gene ID (NCBI) 23067
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SETD1B, also named as KMT2G. Set1B associates with a about 450 kDa complex that contains all five non-catalytic components of the Set1A complex, including CFP1, Rbbp5, Ash2, Wdr5, and Wdr82. The complex is a histone methyltransferase that produces trimethylated histone H3 at Lys4. Set1B is widely expressed, and localize to a largely non-overlapping set of euchromatic nuclear speckles, suggesting that Set1B bind to a unique set of target genes and thus make non-redundant contributions to the epigenetic control of chromatin structure and gene expression( PMID: 17355966). The antibody is specific to SETD1B and, in westernbloting experiments, two specific bands can be detected, about 250 kDa, 450 kDa respectively, in addition, a unknown band around 70 kDa. This antibody is a rabbit polyclonal antibody raised against a region of human SETD1B.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for SETD1B antibody 55005-1-APProtokoll herunterladen
IHC protocol for SETD1B antibody 55005-1-APProtokoll herunterladenl
IF protocol for SETD1B antibody 55005-1-APProtokoll herunterladen
IP protocol for SETD1B antibody 55005-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIF

EMBO J

Histone methyltransferases MLL2 and SETD1A/B play distinct roles in H3K4me3 deposition during the transition from totipotency to pluripotency

Authors - Jingjing Zhang
  • KD Validated
humanWB

Int J Mol Sci

(20S) Ginsenoside Rh2 Exerts Its Anti-Tumor Effect by Disrupting the HSP90A-Cdc37 System in Human Liver Cancer Cells.

Authors - Chen Chen
humanWB,IHC

Am J Cancer Res

Risk model-guided identification of MTDH expression as a marker for ferroptosis induction therapy in head and neck squamous cell carcinoma

Authors - Xueying Wang
mouseIHC

Biogerontology

Histone lysine methyltransferases and their specific methylation marks show significant changes in mouse testes from young to older ages

Authors - Yesim Bilmez
mouseIHC

Histochem Cell Biol

Expression of the histone lysine methyltransferases SETD1B, SETDB1, SETD2, and CFP1 exhibits significant changes in the oocytes and granulosa cells of aged mouse ovaries.

Authors - Yesim Bilmez
mouseWB,IHC,ChIP

Int Immunopharmacol

NF-κB p65 and SETDB1 expedite lipopolysaccharide-induced intestinal inflammation in mice by inducing IRF7/NLR-dependent macrophage M1 polarization

Authors - Li Chen