SETD1B Polyklonaler Antikörper

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• KD/KO Validated

SETD1B Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55005-1-PBS

Synonyme

EC:2.1.1.364, Histone-lysine N-methyltransferase SETD1B, hSET1B, KIAA1076, KMT2G

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  WB analysis of human brain using 55005-1-AP (same clone as 55005-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55005-1-AP (SETD1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 55005-1-AP (same clone as 55005-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55005-1-AP (SETD1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 55005-1-AP (same clone as 55005-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55005-1-AP (SETD1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 55005-1-AP (same clone as 55005-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55005-1-AP (SETD1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 55005-1-AP (same clone as 55005-1-PBS)

  IP result of anti-SETD1B (IP:55005-1-AP, 5ug; Detection:55005-1-AP 1:300) with MCF-7 cells lysate 3250ug. This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 55005-1-AP (same clone as 55005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 55005-1-AP (same clone as 55005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 55005-1-AP (same clone as 55005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 55005-1-AP (SETD1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55005-1-AP (same clone as 55005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55005-1-AP (same clone as 55005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55005-1-AP (same clone as 55005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 55005-1-AP (same clone as 55005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55005-1-AP (same clone as 55005-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 55005-1-AP (same clone as 55005-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SETD1B antibody (55005-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 55005-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55005-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA SETD1B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SET domain containing 1B
• Berechnetes Molekulargewicht: 209 kDa
• Beobachtetes Molekulargewicht: 250-260 kDa, 450 kDa
• GenBank-Zugangsnummer: NM_015048
• Gene symbol: SETD1B
• Gene ID (NCBI): 23067
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SETD1B, also named as KMT2G. Set1B associates with a about 450 kDa complex that contains all five non-catalytic components of the Set1A complex, including CFP1, Rbbp5, Ash2, Wdr5, and Wdr82. The complex is a histone methyltransferase that produces trimethylated histone H3 at Lys4. Set1B is widely expressed, and localize to a largely non-overlapping set of euchromatic nuclear speckles, suggesting that Set1B bind to a unique set of target genes and thus make non-redundant contributions to the epigenetic control of chromatin structure and gene expression( PMID: 17355966). The antibody is specific to SETD1B and, in westernbloting experiments, two specific bands can be detected, about 250 kDa, 450 kDa respectively, in addition, a unknown band around 70 kDa. This antibody is a rabbit polyclonal antibody raised against a region of human SETD1B.