SETD3 Polyklonaler Antikörper

SETD3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28692-1-PBS

Synonyme

C14orf154, SET domain containing 3

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  WB analysis using 28692-1-AP (same clone as 28692-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28692-1-AP (SETD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 28692-1-AP (same clone as 28692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28692-1-AP (SETD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 28692-1-AP (same clone as 28692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28692-1-AP (SETD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 28692-1-AP (same clone as 28692-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SETD3 antibody (28692-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 28692-1-AP (same clone as 28692-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SETD3 antibody (28692-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28692-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SETD3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SETD3 fusion protein Ag30017
• Vollständiger Name: SET domain containing 3
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: NM_032233
• Gene symbol: SETD3
• Gene ID (NCBI): 84193
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SETD3 (SET domain protein 3), a ubiquitously expressed protein, is epigenetically up-regulated in response to muscle loading and hypertrophy. The SETD3 mRNA is ubiquitously expressed at a similar basal level in most examined tissues, with the noticeable exception of the skeletal muscle, kidneys, and testes (PMID: 34685411). SETD3 deficient female mice have severely decreased litter sizes due to primary maternal dystocia that is refractory to ecbolic induction agents (PMID: 30626964).