SETD7 Polyklonaler Antikörper

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• KD/KO Validated

SETD7 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24840-1-PBS

Synonyme

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  WB analysis using 24840-1-AP (same clone as 24840-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24840-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 24840-1-AP (same clone as 24840-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24840-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 24840-1-AP (same clone as 24840-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24840-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 24840-1-AP (same clone as 24840-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24840-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 24840-1-AP (same clone as 24840-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 24840-1-AP (SETD7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24840-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24840-1-PBS bindet in WB, IF/ICC, Indirect ELISA SETD7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SETD7 fusion protein Ag18219
• Vollständiger Name: SET domain containing (lysine methyltransferase) 7
• Berechnetes Molekulargewicht: 366 aa, 41 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC121055
• Gene symbol: SETD7
• Gene ID (NCBI): 80854
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SET domain containing lysine methyltransferase 7 (SETD7, also called SET7/9) was the first lysine methyltransferase (KMT) discovered to specifically monomethylate lysine-4 of histone 3 (H3K4me1) (PMID: 26435321). SETD7 has been shown to have a very broad target specificity in vitro, including transcriptional regulators such as TAF10, p53, ER, p65, STAT3, Rb, Mypt, Tat, and Foxo3 (PMID: 25136132, 30013796).