SETD8 Polyklonaler Antikörper

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SETD8 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14063-1-PBS

Synonyme

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  WB analysis using 14063-1-AP (same clone as 14063-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14063-1-AP (SETD8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 14063-1-AP (same clone as 14063-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 14063-1-AP (SETD8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 14063-1-AP (same clone as 14063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14063-1-AP (SETD8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 14063-1-AP (same clone as 14063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14063-1-AP (SETD8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 14063-1-AP (same clone as 14063-1-PBS)

  1x10^6 HEK-293T cells were intracellularly stained with 0.4 ug SETD8 Polyclonal antibody (14063-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14063-1-PBS bindet in WB, IHC, Indirect ELISA SETD8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SETD8 fusion protein Ag5114
• Vollständiger Name: SET domain containing (lysine methyltransferase) 8
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC050346
• Gene symbol: SETD8
• Gene ID (NCBI): 387893
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SETD8, also known as Set8, PR-Set7, or KMT5A, is a member of the SET domain family of histone lysine methyltransferases and, to date, the only known enzyme that catalyzes monomethylation of H4K20 in eukaryotes, which is involved in DNA damage response, mitotic condensation, and DNA replication. SETD8 is overexpressed in various types of tumors, such as bladder cancer, non-small cell lung carcinoma, and small cell lung carcinoma, and is associated with a shorter survival time for gastric, esophageal, and prostate cancer patients.