SETDB1 Monoklonaler Antikörper

SETDB1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1H6E5

Kat-Nr. : 66293-1-Ig

Synonyme

Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, ESET, ERG-associated protein with SET domain, EC:2.1.1.366

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  WB analysis using 66293-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66293-1-Ig (SETDB1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 66293-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66293-1-Ig (SETDB1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66293-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66293-1-Ig (SETDB1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 66293-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66293-1-Ig (SETDB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66293-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66293-1-Ig (SETDB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66293-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66293-1-Ig (SETDB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66293-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66293-1-Ig (SETDB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 66293-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66293-1-Ig (SETDB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 66293-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66293-1-Ig (SETDB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 66293-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SETDB1 antibody (66293-1-Ig, Clone: 1H6E5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, humanes Herzgewebe, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, Maus-Kolongewebe, Ratten-Kolongewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66293-1-Ig bindet in WB, IHC, IF/ICC, ELISA SETDB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SETDB1 fusion protein Ag21644
• Vollständiger Name: SET domain, bifurcated 1
• Berechnetes Molekulargewicht: 143 kDa
• Beobachtetes Molekulargewicht: 170-180 kDa
• GenBank-Zugangsnummer: BC009362
• Gene symbol: SETDB1
• Gene ID (NCBI): 9869
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa (PMID: 11791185).