SETDB1 Rekombinanter Antikörper

SETDB1 Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240805F2

Kat-Nr. : 83772-6-PBS

Synonyme

Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, ESET, ERG-associated protein with SET domain, EC:2.1.1.366

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  IHC staining of human colon cancer using 83772-6-RR (same clone as 83772-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83772-6-RR (SETDB1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83772-6-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83772-6-RR (same clone as 83772-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SETDB1 antibody (83772-6-RR, Clone: 240805F2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83772-6-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83772-6-RR (same clone as 83772-6-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SETDB1 Recombinant antibody (83772-6-RR, Clone:240805F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83772-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83772-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83772-6-RR against Human SETDB1 were performed. The affinity constant is 0.18 nM.

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Geprüfte Anwendungen

Produktinformation

83772-6-PBS bindet in IHC, IF/ICC, FC (Intra), ELISA SETDB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SETDB1 fusion protein Ag1725
• Vollständiger Name: SET domain, bifurcated 1
• Berechnetes Molekulargewicht: 143 kDa
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: BC009362
• Gene symbol: SETDB1
• Gene ID (NCBI): 9869
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SETDB1.The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa (PMID: 11791185).