SF1 Polyklonaler Antikörper

SF1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11635-1-PBS

Synonyme

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  WB analysis using 11635-1-AP (same clone as 11635-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11635-1-AP (SF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11635-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 11635-1-AP (same clone as 11635-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11635-1-AP (SF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11635-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse ovary using 11635-1-AP (same clone as 11635-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11635-1-AP (SF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11635-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11635-1-PBS bindet in WB, IHC, Indirect ELISA SF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SF1 fusion protein Ag2209
• Vollständiger Name: splicing factor 1
• Berechnetes Molekulargewicht: 638 aa, 68 kDa
• Beobachtetes Molekulargewicht: 65-71 kDa
• GenBank-Zugangsnummer: BC020217
• Gene symbol: SF1
• Gene ID (NCBI): 7536
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SF1 is a nuclear pre-mRNA splicing factor, it specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The molecular weight of SF1 ranges from 59 kDa to 71 kDa due to alternative splicing.